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licA [2019-08-12 13:12:10]
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licA [2019-08-12 13:12:10]

lichenan-specific permease of the [SW|phosphotransferase systems|phosphotransferase system], EIIA of the [category|SW 1.2.2|PTS]
locus
BSU_38570
pI
4.64
mw
12.04 kDa
protein length
110 aa Sequence Blast
gene length
333 bp Sequence Blast
function
lichenan uptake and phosphorylation
product
lichenan-specific [category|SW 1.2.2|PTS], EIIA component
essential
no
synonyms
celC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    3,959,841 → 3,960,173

    The protein

    Catalyzed reaction/ biological activity

  • Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

    • Protein family

  • [category|SW 1.2.2|PTS] permease, lactose family [Pubmed|10627040]

    • Paralogous protein(s)

  • [protein|44014896157718D3A6190DFDBD663C5CB9DA63FC|GmuA]

    • Structure

  • [PDB|3K1S] (IIA(Cel) from ''B. anthracis'', 42% identity)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|65FC7F4ED92F747DB7BC5672DCA2D2320A471134|licB]-[gene|83A7C160B0301A2B51B65478D99ACB753D67AF74|licC]-[gene|E8B6C8E722BEE866878CDD816B7828C132F94FCD|licA]-[gene|84D8AA0AFBF46273F6B4198B49440D55470FEC0F|licH]
    description
    [Pubmed|8990303]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8990303], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|8990303], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E087ABA705CE94E2CAD44A0F2FB265B0F7B04399|LicR]: activation, [Pubmed|8990303], in [regulon|E087ABA705CE94E2CAD44A0F2FB265B0F7B04399|LicR regulon]

    • regulation

  • carbon catabolite repression ([protein|search|CcpA]) [Pubmed|8990303]
    • view in new tab

    Biological materials

    Mutant

  • BKE38570 (Δ[gene|E8B6C8E722BEE866878CDD816B7828C132F94FCD|licA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTCACGCGATTCACTTCCT, downstream forward: _UP4_ACCGAGCAAAGGGGAGCAAG
  • BKK38570 (Δ[gene|E8B6C8E722BEE866878CDD816B7828C132F94FCD|licA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTCACGCGATTCACTTCCT, downstream forward: _UP4_ACCGAGCAAAGGGGAGCAAG

    • References

  • 19087206,8990303,16872404,10438772,10627040,8990303,27766092